BluSelect™ 优势:含有X-Gal 和IPTG的即用型溶液。
操作步骤:
只需取100ul,涂到平板上即可。
或者取100ul BluSelect 加入25ml 低于45˚C 的Agar溶液,倒入平板即可。
另有添加各种抗生素的筛选溶液(可根据客户需要配制不同抗生素筛选液),请参考BluSelectTM Select
与传统方法相比较请参考下表和图片
Features |
BluSelect™ |
home-made |
Preparation |
Ready-to-use Simply pour 100 µl on a Petri dish |
Need to be weighed & dissolve |
Stability as a solution |
24 months at 4˚C 12 months at RT |
1 day at RT or 4˚C |
Toxicity |
Safe and non-toxic for the user and the environment |
DMF: Toxic and explosive DMSO: Cancer agent |
IPTG |
Formulation contains IPTG |
Need to be weighed & dissolve as a separate solution. |
Antibiotic resistance selection |
Can be formulated with most utilized antibiotics |
Need to be added separately |
Waiting time before spreading bacteria |
Dries quickly |
Up to 30 minutes |
User friendly |
One solution, one step. User Friendly |
3 solutions, 3 steps |
Intensity of coloration / sensitivity of detection |
Enhanced blue coloration Particularly suitable for low copy vectors such as BAC Better discrimination between white and blue colonies |
Need to refrigerate plates at 4°C to enhance blue color |
X-Gal用于蓝白斑筛选,用于细菌克隆探测LacZ基因。蓝白斑筛选是用于检测DNA片段克隆的标准方法,是基因组学、蛋白质组学、分子生物学研究中,应用较广泛的一种方法。
Product |
X-Gal Conc. |
IPTG Conc. |
Antibiotic Conc. |
BluSelect volume per petri dish |
BluSelect |
10 mg/ml |
8 mg/ml |
0 mg/ml |
100ul |